應用文集

The 60 SPD method has a 21 minute gradient and a cycle time of 24 minutes. The analytical column is equilibrated at 2 µl/min. The gradient flow is 1 µl/min and increased to 2 µl/min for washing (Figure 1). Two columns can be used for the method, either the EV1064 Endurance column at ambient temperature (23 °C in this case) or the EV1109 Performance column, which should be used at 40 °C. Both columns should be used in connection with the appropriate emitter