應用文集

An automated protocol was developed to perform magnetic bead-based immobilized metal affinity chromatography (IMAC) on the Opentrons OT-2 liquid handler. We tested the protocol using the Pierce™ Ni NTA Magnetic Agarose Beads (Thermo Fisher Scientific, Waltham, MA, USA) for extracting the protein of interest (His-tagged GAPDH) on the OT-2 using samples prepared by diluting recombinant GAPDH protein in lysis buffer, HEp-2 cell lysates, and bacterial cells