應用文集

Doping control laboratories constantly look for technological improvements providing addition al sensitivity, while maintaining the needed high throughput to perform several thousands of samples per year. This is particularly relevant to ensure the accurate identification of banned compounds such as synthetic anabolic androgenic steroids (AAS) at lower limits of detection as well as extending the period after usage, where the compound is detectable in the analysis. We have previously described the sensitive detection of protein-based banned substances such as EPO with the Evosep One1,2. Since AAS are significantly more hydro phobic than the majority of tryptic peptides, we generated a new Evosep method (High Organic method) to efficiently detect stanozolol, a notorious synthetic AAS. This method allows the sequential elution of analytes within a nearly linear 0 – 100 % ACN gradient with a through put of 100 samples per day.