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The field of proteomics is being rapidly rede fined by technological advancements towards increasingly robust and faster instrumentation. This development is driving a new era where the combination of high-throughput and excel lent performance paves the way for novel proteomics applications across various fields. To address this, we have established the framework for a versatile, modular, and fully automated sample preparation strategy based on magnetic beads integrated with Evotip Pure sample loading. Here we present the workflow on the Opentrons OT-2 liquid handler (OT-2) for efficient and robust preparation of ready-to-ana lyze peptides from extracted proteins with just 4 hours of digestion at room temperature. For ease-of-use, we have designed a HTML-based user inter face that allows for easy design of experiments, and produces a Python script that can be directly imported into the Opentrons app. The unique properties of the Evotip Pure allow for immediate and online desalting and storage of digested peptides for a lossless integration between sample preparation and LC-MS analysis. This framework presents a solid foundation on which functional modules for versatile proteomics applications can be added in the future (Figure 1).